HPCE-512 by deltaDOT
Improved Discovery Power – no molecular labels
deltaDOT HPCE-512 offers dramatically improved discovery power in comparison to other conventional CE systems without the use of molecular labels.
This is achieved by the combination of substantially enhanced resolution, sensitivity and quantification power of the instrument, along with the digital output of data delivered as clearly resolved separations. The system has proven capability in almost all modes of CE.
It allows analyte molecular weight and concentration to be assigned and absolute measurements can performed using calibrating markers. Supplied with the instrument is a powerful customized.
UV light is directed from the source via filters and optical components at the capillary window. The detector assembly contains a photodiode array of 512 detection points.
For most electrophoretic separations a fused silica capillary coated with polyimide on the outside is used. The separation lengths can be set from 20 cm to 70 cm by selecting the appropriate route in the capillary block.
The data analysis of biomolecules performed using the HPCE-412 is enabled by proprietary Equiphase Vertexing Algorithm (EVA) and Generalised Separation Transform (GST) algorithms. GST is a method of combining the data from the multiple pixels in a natural way which preserves the peak shape information of the electropherograms while at the same time maximising the signal-to-noise ratio. A factor of 10 increase in signal-to-noise using GST as compared to using single point detection, is typically observed.
For details of the data that the HPCE-512 can generate please visit Application Note Downloads.