Capillary Electrophoresis Based Analysis
deltaDOT’s team of scientists has developed a considerable expertise in CE based analysis and in solving clients’ analytical problems. Using our own HPCE LFII® analytical instruments we also offer Analytical Services, Contract Research and Scientific Collaboration to industry and academia from our laboratories at the London Bioscience Innovation Centre. Our recent and current collaboration examples include those shown below.
Contract Research and Scientific Collaboration
Using our own HPCE LFII® analytical instruments we also offer Analytical Services, Contract Research and Scientific Collaboration to industry and academia from our laboratories at the London Bioscience Innovation Centre. Our recent and current collaboration examples include those shown listed below.
Glomerular filtration rate (GFR) by Iohexol clearance
Designed to identify early renal disease and monitor its progression in companion animals. High Performance Capillary Electrophoresis (HPCE) method is used for the determination of GFR values by administered with the contrast agent Iohexol (Omnipaque™). This capillary electrophoresis technique gives quicker results, with minimal sample preparation and lower costs compared to traditional HPLC techniques. The method allows next-day estimation of feline GFR in veterinary practice using as few as 3 samples taken after injection of an Iohexol bolus (more on Apps page).
CZE separation of amino acid mixtures for neo-natal screening for inborn errors of metabolism, IVF applications. Capillary electrophoresis using indirect UV detection is a fast and convenient method for the analysis of amino acids, compared to HPLC. A reversed polarity mode is employed, with the sample injected at the cathode and electrophoresed
Analysis of both the heavy and light chains of antibodies, including glycosylation analysis on both. Both CGE analysis (for molecular weight determination and PTM analysis) and CZE analysis (for antibody-antigen interactions) can be carried out.
Viral Titer (deltaTITER) – kit-based baculovirus titration methodology developed jointly with GSK. Fast and accurate titration – 18 samples can be processed in one hour and results obtained overnight. Repeatability of titrations <10% compared with 50% obtained for traditional plaque assays.
Diagnostics, therapeutics, formulation and QA/QC studies. Used in the identification of recombinant vs. naturally occurring erythropoietin (Epo). Could be used as a test for doping in athletes. Potential use also in monitoring drug dose of patients receiving recombinant EPO for end stage renal failure. Transferrin glycoform analysis. Used in the identification of carbohydrate deficient transferrin in alcoholic patients.