Rugged and simplified systems
The majority of deltaDOTs technology is applied in the field of capillary based electrophoresis, but the company has also extensive experience in microfluidics, which can enable analyses to be performed in systems potentially the size of a laptop, with separation media the size of a credit card. This outline proposal will introduce the application of LFII® technologies to these portable microfluidic devices and show how the label free analytical approach will play a key part in the rapid response to both pandemic, frontline threat identification and bioterror incidents. This project will produce a compact, ruggedized LFII® based device capable of field portability by semi-skilled operatives in stressful in-theatre frontline conditions. The system will be designed from the start as a solution to the end-user's need for a cost effective, reliable and tough DNA sequencer.
PEREGRINE 96
deltaDOT has manufactured two prototype PEREGRINE96 instruments each capable of processing samples loaded in a temperature controlled 96 well microtitre plate. The system utilizes innovative technologies employed within the commercialized PEREGRINE I system, combined with a bespoke motion handling system, developed by Innovations Biotech, to provide the program with a LFII® capillary electrophoresis platform with a standardized 96 well microtiter plate front end format, reducing the time between operation steps and increasing throughput over the conventional system due to optimized motion handling.I n addition to the development of the two prototype PEREGRINE96 systems, deltaDOT have designed and developed bespoke control software to control and acquire data in real time from the system and analysis software responsible for processing and manipulating the data generated, by applying deltaDOT's proprietary analysis algorithms; specifically the Equiphase Vertexing Algorithm (EVA) and the Generalized Separation Transform (GST).
